The Best Places to Buy NGS Library Prep Kits Online and In-Store

Explore next-generation sequencing library prep kits that allow scientists to ask virtually any question related to the genome, transcriptome, or epigenome of any organism. Optimized for Illumina sequencing platforms, our library prep kits support a large range of throughput needs and sample types while boosting workflow efficiency.

You will get efficient and thoughtful service from TSKT.

Ligation Sequencing Kit V14 SQK-LSK114

A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) and long read singleplex samples.

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: ng dsDNA, 100+ ng DNA if performing fragmentation or PCR,100-200 fmol of  amplicons or cDNA

Ligation Sequencing Kit XL V14 SQK-LSK114-XL

A versatile sequencing kit optimised for modal raw read accuracy of Q20+ (99%+) with long read singleplex samples. Multiple samples can be processed simultaneously.

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: = fragment length
• Input amount: ng dsDNA, 100+ ng DNA if performing fragmentation or PCR, 100-200 fmol of  amplicons or cDNA

Native Barcoding Kit 24 V14 SQK-NBD114.24

A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This uses our latest Kit 14 chemistry

• Preparation time: 140 minutes
• Read length: = fragment length
• Input amount: 400- ng for gDNA. 200 fmol (130 ng for 1 kb amplicons) for amplicon input

Native Barcoding Kit 96 V14 SQK-NBD114.96

A versatile method of preparing barcoded sequencing libraries optimised for modal raw read accuracy of Q20+ (99%+) and long read multiplexed samples.

This uses our latest Kit 14 chemistry

• Preparation time: 140 minutes
• Read length: = to fragment length
• Input amount: 400 ng per sample of gDNA, 200 fmol per sample (130 ng for 1 kb amplicons)

Ultra-Long DNA Sequencing Kit V14 SQK-ULK114

An overnight method to prepare ultra-high molecular weight DNA for sequencing ultra-long reads (N50 >50 kb).

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: ~200 minutes + overnight elution
• Read length: N50 >50 kb
• Input amount: 6 million cells

Rapid Barcoding Kit 24 V14 SQK-RBK114.24

Simple and rapid library preparation, with barcoding for up to 24 gDNA samples.

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 200 ng dsDNA per sample

Rapid Barcoding Kit 96 V14 SQK-RBK114.96

Simple and rapid library preparation, with barcoding for up to 96 gDNA samples.

This uses our latest Kit 14 chemistry

• Preparation time: 60 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 200 ng dsDNA per sample

Rapid Sequencing Kit V14 SQK-RAD114

A simple and rapid library preparation method for genomic DNA optimised for speed with improved modal raw read accuracy.

This is an Early Access product

This uses our latest Kit 14 chemistry

• Preparation time: 10 minutes
• Read length: Random distribution, dependent on input fragment length
• Input amount: 100 ng dsDNA

Rapid PCR Barcoding Kit 24 V14 SQK-RPB114.24

Rapid and simple method of preparing up to 24 barcoded samples for sequencing from 1-5 ng of gDNA using PCR.

This is an Early Access product

Contact us to discuss your requirements of NGS Library Prep Kits. Our experienced sales team can help you identify the options that best suit your needs.

This uses our latest Kit 14 chemistry

• Preparation time: 15 minutes + PCR
• Read length: 4-5 kb
• Input amount: 1-5 ng gDNA per sample

16S Barcoding Kit 24 V14 SQK-16S114.24

Genus-level bacterial identification with barcoding for up to 24 samples.

Product lead time: 1 week

This uses our latest Kit 14 chemistry.

• Preparation time: 25 minutes + PCR
• Read length: Full-length 16S gene (~1.5 kb)
• Input amount: 10 ng gDNA per sample

Multiplex Ligation Sequencing Kit XL V14 SQK-MLK114.96-XL

A sequencing kit optimised for high output and PCR-free low-plex sequencing of 2-3 samples for Whole Genome Sequencing (WGS).

This uses our latest Kit 14 chemistry

Product lead time: 1 week

• Preparation time: 150 minutes
• Read length: = Fragment length
• Input amount: ng dsDNA per sample

Direct RNA Sequencing Kit SQK-RNA004

A sequencing kit optimised for sequencing native RNA with improved output and accuracy.

Product lead time: 1 week

• Preparation time: 135 minutes
• Read length: = RNA length
• Input amount: 1 μg total RNA, 300 ng poly(A)+ RNA

Direct RNA Sequencing Kit XL SQK-RNA004-XL

A sequencing kit optimised for sequencing native RNA with improved output and accuracy. Multiple samples can be processed simultaneously.

This is an Early Access product.

Product lead time: 1 week

• Preparation time: ~135 minutes
• Read length: = RNA length
• Input amount: 300 ng poly(A) tailed RNA or 1 μg total RNA

cDNA-PCR Sequencing Kit V14 SQK-PCS114

A sequencing kit optimised for identification and quantification of full-length transcripts with highest output.

This is an Early Access product

Product lead time: 2 weeks

This uses our latest Kit 14 chemistry.

• Preparation time: 225 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 10 ng poly(A)+ RNA or 500 ng total RNA

cDNA-PCR Barcoding Kit V14 SQK-PCB114.24

A sequencing kit optimised for identification and quantification of full-length transcripts with highest output, including barcodes for up to 24 samples.

This is an Early Access product

Product lead time: 2 weeks

This uses our latest Kit 14 chemistry.

For more information, please visit Immunology Kits.

• Preparation time: 225 minutes + PCR
• Read length: Enriched for full-length cDNA during PCR
• Input amount: 10 ng poly(A)+ RNA or 500 ng total RNA per sample

COVID Mini V14: 576 samples C19MINI114